Thanks to funding from the Canadian Foundation for Innovation (CFI), the Nova Scotia Research and Innovation Trust (NSRIT), and “in kind” contributions from companies/suppliers, we have been able to develop a state of the art genetic profiling and analysis facility. Information on some of our key pieces of equipment is below.
All of our work is based on DNA fragment analysis and/or DNA sequencing. Therefore, the centrepiece of our facility is a capillary electrophoresis and analysis system. For this, we are using an ABI 3500xl, which has 24 capillaries. This is ideal for us because it is small enough to make protocol development and optimization efficient, but is also capable of high-throughput profiling.
The thermal cyclers that we are using are the ABI Veriti 96-well thermal cyclers. These are great because instead of a temperature gradient, you can specifically program 6 different annealing temperatures within the run. This is ideal for us because a lot of our work involves protocol development and optimization prior to conducting high-throughput genotyping.
We have a MacPro server that serves two primary purposes. First, it is the server that houses our databases, lab books, protocols, etc. Second, we us it remotely for high performance computing. When we really need high-performance computational power, we also have access to the ACENET system.